{
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  "title": "AlphaFold-Multimer screen predicted Tmem81 as a fertilization factor, confirmed in zebrafish and mice",
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  "account": "In a paper published in Cell on 17 October 2024, the Pauli laboratory and collaborators reported an AlphaFold-Multimer screen. It identified Tmem81 as part of a conserved trimeric sperm complex with the known essential fertilization factors Izumo1 and Spaca6. The prediction was then tested at the bench. The authors demonstrated that Tmem81 is essential for male fertility in zebrafish and mice. They showed Izumo1, Spaca6 and Tmem81 interact in zebrafish sperm and the human orthologues interact in vitro. They found complex formation creates the binding site for the egg factor Bouncer in zebrafish. The screen and the validation were performed independently of AlphaFold's developer.",
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  "purpose": "Predict protein-protein complexes in order to find missing components of the vertebrate sperm-egg interaction machinery.",
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      "citation": "Deneke VE, Blaha A, Lu Y, et al. (2024). A conserved fertilization complex bridges sperm and egg in vertebrates. Cell.",
      "title": "A conserved fertilization complex bridges sperm and egg in vertebrates",
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